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KMID : 0352819950100020001
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Kosin Medical Journal
1995 Volume.10 No. 2 p.1 ~ p.8
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Department of Urology, The University of Texas M.D. Anderson Cancer Center Natural
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Andrew C. von Eschenbach
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Abstract
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Natural killer (NK) cells that had infilterated renal cell carcinoma(RCC) proliferated vigorously in culture which activated interleukin-2(IL-2) and lysed autologous tumorcells. We studied the susceptibility of RCC cells to NK-cell lysis and
their
ability to stimulate proliferation and function of NK cells. Cells from primary culture of RCC(p-RCC cells) were significantly more susceptible to lysis mediated by human NK3.3 clones than were cells from primary cultue of metastatic melanomas.
RCC
cells clones was also susceptible to lysis by NK3.3 clones and IL-2 activated peripheral blood lymphocytes(PBLs). Incubation of NK3.3 clones with p-RCC cells in the absence of IL-2 induced proliferation of NK3.3 clones. whereas incubation with
cells
from primary culture of metastatic melanomas, K562 cells tested did not. The p-RCC cells from earlier passages. Cell-free culture supernatants of p-RCC cells with or without NK3.3 clones failed to induce NK-cell proliferation. Incubation of NK
cells
purified from PBLs with p-RCC cells induced higher proliferation of the NK cells only in the presence of IL-2, whereas incubation with cells from primary culture of metastatic melanomas did not. In summary these results suggest that RCC cells are
able
to activate NK cells, potentially through cell-to-cell interaction.
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KEYWORD
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